First
identification A, B, E
Second identification A, C, D, E
A.
Dissolve 0.5 g in 50 ml of water R.
Immediately adds 5ml of hydrochloric acid R1.
Filter and wash the precipitate with water R.
Dry under vacuum at 80oC for 2 hours.
The precipitate obtained melts (2.2.14) at
96°C to 99°C.
B.
Examine the precipitate obtained in
identification test A by infrared absorption
spectrophotometry (2.2.24) comparing with the
spectrum obtained with propyl parahydroxy
benzoate CRS.
C.
Examine the chromatograms obtained in the
test for related substances. The principal spot
in the chromatogram obtained with test solution
(b) is similar in position and size to the
principal spot in the chromatogram obtained with
reference solution (c).
D.
To about 10 mg in a test-tube add 1 ml of
sodium carbonate solution R, boil for 30s and
cool. Add 5 ml of aminopyrazolone solution R and
1 ml of potassium ferricyanide solution R and
mix. An orange to red colour develops.
E.
To 1 ml of Solution S (see Tests)
add 1 ml of water R. The solution gives reaction
(a) of sodium (2.3.1) |
