First identification A, B, E
Second identification A, C, D, E
A.
Dissolve 0.5 g in 50 ml of water R.
Immediately adds 5ml of hydrochloric acid
R1. Filter and wash the precipitate with
water R. Dry under vacuum at 80oC
for 2 hours. The precipitate obtained melts
(2.2.14) at 125°C to 128°C.
B.
Examine the precipitate obtained in
identification test A by infrared absorption
spectrophotometry (2.2.24) comparing with
the spectrum obtained with methyl
parahydroxy benzoate CRS.
C.
Examine the chromatograms obtained in
the test for related substances. The
principal spot in the chromatogram obtained
with test solution (b) is similar in
position and size to the principal spot in
the chromatogram obtained with reference
solution (c).
D.
To about 10 mg in a test-tube add 1
ml of sodium carbonate solution R, boil for
30s and cool. Add 5 ml of aminopyrazolone
solution R and 1 ml of potassium
ferricyanide solution R and mix. An orange
to red colour develops.
E.
To 1 ml of Solution S (see
Tests) add 1 ml of water R. The solution
gives reaction (a) of sodium (2.3.1) |
