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Dissolve 1.0 g in sufficient ethanol (96%) to produce 10 ml. To 2 ml of the solution add 3 ml of ethanol (96%), 5 ml of carbon dioxide-free water and 0.1 ml of Bromocresol green solution .Not more than 0.1 ml of 0.1 N sodium hydroxide VS is required to change the colour of the solution to blue.
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