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A.
The light absorption in the range 230
to 360 nm of a 0.0005% w/v in ethanol (95%)
exhibits a maximum at about 258 nm,
absorbance at about 258 nm, 0.44 to 0.47
B.
To about 10 mg in a test tube add 1 ml
of sodium carbonate solution, heat
to boiling for 30 seconds and cool (solution
A) .To a further 10 mg in a test tube add 1
ml of sodium carbonate solution, the
substance partly dissolves (solution B). Add
at the same time to each of solutions A and
B 5 ml of aminophenazone solution and 1 ml
of potassium ferricyanide solution and mix.
Solution B is yellow to orange brown.
Solution A is orange to Red and the colour
is clearly more intense than any similar
colour that may be obtained with solution B.. |